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Kaneka Corp cationic liposome dac-30
Cationic Liposome Dac 30, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cationic+liposomes+(dac-30/dac+30/pm11972864-46-41-44
Average 90 stars, based on 1 article reviews
cationic liposome dac-30 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Transfection:

Article Title: p21-activated Kinase 1 Activates the Nuclear Factor κB (NF-κB)-inducing Kinase-IκB Kinases NF-κB Pathway and Proinflammatory Cytokines in Helicobacter pyloriInfection
Article Snippet: .. Transient Transfections and Reporter Assays—Transactivating activity of NF-kB was analyzed in 293 or HeLa cells by cotransfection of a luciferase expression plasmid (400 ng) containing three repeats of the NF-kB human immunodeficiency virus-binding site and expression constructs using cationic liposomes (DAC-30, Eurogentec). .. 16 h after transfection cells were infected with H. pylori, treated with 10 ng ml21 tumor necrosis factor a (TNFa) (Promega), or left untreated.

Article Title: Identification of the Receptor Subtype Involved in the Analgesic Effect of Neurotensin
Article Snippet: .. Stable expression of the NTR2 into CHO cells was obtained by transfection with 5 mg of recombinant pcDNA3 vector using 25 mg of the cationic liposome DAC-30 (Eurogentec) according to the manufacturer’s recommendations. .. Clonal cell lines were selected by Geneticin (Sigma) (0.5 mg/ml) and isolated before determination of expression level.

Article Title: Helicobacter pylori activates the histidine decarboxylase promoter through a mitogen-activated protein kinase pathway independent of pathogenicity island-encoded virulence factors.
Article Snippet: .. Transient transfections of 1–2 mg of reporter constructs were carried out using cationic liposomes (Dac-30, Eurogentec, Sart Tilman, Belgium) according to a protocol reported previously (13, 52). ..

Activity Assay:

Article Title: p21-activated Kinase 1 Activates the Nuclear Factor κB (NF-κB)-inducing Kinase-IκB Kinases NF-κB Pathway and Proinflammatory Cytokines in Helicobacter pyloriInfection
Article Snippet: .. Transient Transfections and Reporter Assays—Transactivating activity of NF-kB was analyzed in 293 or HeLa cells by cotransfection of a luciferase expression plasmid (400 ng) containing three repeats of the NF-kB human immunodeficiency virus-binding site and expression constructs using cationic liposomes (DAC-30, Eurogentec). .. 16 h after transfection cells were infected with H. pylori, treated with 10 ng ml21 tumor necrosis factor a (TNFa) (Promega), or left untreated.

Article Title: COP9 signalosome-directed c-Jun activation/stabilization is independent of JNK.
Article Snippet: .. Transactivating activity of AP-1 was measured at 50–70% confluence by cotransfection of a luciferase expression plasmid containing three repeats of the AP-1 binding site and other expression constructs using cationic liposomes (DAC-30, Eurogentec, Sart Tilman, Belgium). .. Expression constructs Sgn5, Sgn2, and Dc-Jun-(1–226) were cloned into the pcDNA3 vector (Invitrogen) expressing a N-terminal Flag-tagged sequence.

Cotransfection:

Article Title: p21-activated Kinase 1 Activates the Nuclear Factor κB (NF-κB)-inducing Kinase-IκB Kinases NF-κB Pathway and Proinflammatory Cytokines in Helicobacter pyloriInfection
Article Snippet: .. Transient Transfections and Reporter Assays—Transactivating activity of NF-kB was analyzed in 293 or HeLa cells by cotransfection of a luciferase expression plasmid (400 ng) containing three repeats of the NF-kB human immunodeficiency virus-binding site and expression constructs using cationic liposomes (DAC-30, Eurogentec). .. 16 h after transfection cells were infected with H. pylori, treated with 10 ng ml21 tumor necrosis factor a (TNFa) (Promega), or left untreated.

Article Title: COP9 signalosome-directed c-Jun activation/stabilization is independent of JNK.
Article Snippet: .. Transactivating activity of AP-1 was measured at 50–70% confluence by cotransfection of a luciferase expression plasmid containing three repeats of the AP-1 binding site and other expression constructs using cationic liposomes (DAC-30, Eurogentec, Sart Tilman, Belgium). .. Expression constructs Sgn5, Sgn2, and Dc-Jun-(1–226) were cloned into the pcDNA3 vector (Invitrogen) expressing a N-terminal Flag-tagged sequence.

Luciferase:

Article Title: p21-activated Kinase 1 Activates the Nuclear Factor κB (NF-κB)-inducing Kinase-IκB Kinases NF-κB Pathway and Proinflammatory Cytokines in Helicobacter pyloriInfection
Article Snippet: .. Transient Transfections and Reporter Assays—Transactivating activity of NF-kB was analyzed in 293 or HeLa cells by cotransfection of a luciferase expression plasmid (400 ng) containing three repeats of the NF-kB human immunodeficiency virus-binding site and expression constructs using cationic liposomes (DAC-30, Eurogentec). .. 16 h after transfection cells were infected with H. pylori, treated with 10 ng ml21 tumor necrosis factor a (TNFa) (Promega), or left untreated.

Article Title: COP9 signalosome-directed c-Jun activation/stabilization is independent of JNK.
Article Snippet: .. Transactivating activity of AP-1 was measured at 50–70% confluence by cotransfection of a luciferase expression plasmid containing three repeats of the AP-1 binding site and other expression constructs using cationic liposomes (DAC-30, Eurogentec, Sart Tilman, Belgium). .. Expression constructs Sgn5, Sgn2, and Dc-Jun-(1–226) were cloned into the pcDNA3 vector (Invitrogen) expressing a N-terminal Flag-tagged sequence.

Expressing:

Article Title: p21-activated Kinase 1 Activates the Nuclear Factor κB (NF-κB)-inducing Kinase-IκB Kinases NF-κB Pathway and Proinflammatory Cytokines in Helicobacter pyloriInfection
Article Snippet: .. Transient Transfections and Reporter Assays—Transactivating activity of NF-kB was analyzed in 293 or HeLa cells by cotransfection of a luciferase expression plasmid (400 ng) containing three repeats of the NF-kB human immunodeficiency virus-binding site and expression constructs using cationic liposomes (DAC-30, Eurogentec). .. 16 h after transfection cells were infected with H. pylori, treated with 10 ng ml21 tumor necrosis factor a (TNFa) (Promega), or left untreated.

Article Title: COP9 signalosome-directed c-Jun activation/stabilization is independent of JNK.
Article Snippet: .. Transactivating activity of AP-1 was measured at 50–70% confluence by cotransfection of a luciferase expression plasmid containing three repeats of the AP-1 binding site and other expression constructs using cationic liposomes (DAC-30, Eurogentec, Sart Tilman, Belgium). .. Expression constructs Sgn5, Sgn2, and Dc-Jun-(1–226) were cloned into the pcDNA3 vector (Invitrogen) expressing a N-terminal Flag-tagged sequence.

Article Title: Identification of the Receptor Subtype Involved in the Analgesic Effect of Neurotensin
Article Snippet: .. Stable expression of the NTR2 into CHO cells was obtained by transfection with 5 mg of recombinant pcDNA3 vector using 25 mg of the cationic liposome DAC-30 (Eurogentec) according to the manufacturer’s recommendations. .. Clonal cell lines were selected by Geneticin (Sigma) (0.5 mg/ml) and isolated before determination of expression level.

Plasmid Preparation:

Article Title: p21-activated Kinase 1 Activates the Nuclear Factor κB (NF-κB)-inducing Kinase-IκB Kinases NF-κB Pathway and Proinflammatory Cytokines in Helicobacter pyloriInfection
Article Snippet: .. Transient Transfections and Reporter Assays—Transactivating activity of NF-kB was analyzed in 293 or HeLa cells by cotransfection of a luciferase expression plasmid (400 ng) containing three repeats of the NF-kB human immunodeficiency virus-binding site and expression constructs using cationic liposomes (DAC-30, Eurogentec). .. 16 h after transfection cells were infected with H. pylori, treated with 10 ng ml21 tumor necrosis factor a (TNFa) (Promega), or left untreated.

Article Title: COP9 signalosome-directed c-Jun activation/stabilization is independent of JNK.
Article Snippet: .. Transactivating activity of AP-1 was measured at 50–70% confluence by cotransfection of a luciferase expression plasmid containing three repeats of the AP-1 binding site and other expression constructs using cationic liposomes (DAC-30, Eurogentec, Sart Tilman, Belgium). .. Expression constructs Sgn5, Sgn2, and Dc-Jun-(1–226) were cloned into the pcDNA3 vector (Invitrogen) expressing a N-terminal Flag-tagged sequence.

Virus:

Article Title: p21-activated Kinase 1 Activates the Nuclear Factor κB (NF-κB)-inducing Kinase-IκB Kinases NF-κB Pathway and Proinflammatory Cytokines in Helicobacter pyloriInfection
Article Snippet: .. Transient Transfections and Reporter Assays—Transactivating activity of NF-kB was analyzed in 293 or HeLa cells by cotransfection of a luciferase expression plasmid (400 ng) containing three repeats of the NF-kB human immunodeficiency virus-binding site and expression constructs using cationic liposomes (DAC-30, Eurogentec). .. 16 h after transfection cells were infected with H. pylori, treated with 10 ng ml21 tumor necrosis factor a (TNFa) (Promega), or left untreated.

Construct:

Article Title: p21-activated Kinase 1 Activates the Nuclear Factor κB (NF-κB)-inducing Kinase-IκB Kinases NF-κB Pathway and Proinflammatory Cytokines in Helicobacter pyloriInfection
Article Snippet: .. Transient Transfections and Reporter Assays—Transactivating activity of NF-kB was analyzed in 293 or HeLa cells by cotransfection of a luciferase expression plasmid (400 ng) containing three repeats of the NF-kB human immunodeficiency virus-binding site and expression constructs using cationic liposomes (DAC-30, Eurogentec). .. 16 h after transfection cells were infected with H. pylori, treated with 10 ng ml21 tumor necrosis factor a (TNFa) (Promega), or left untreated.

Article Title: COP9 signalosome-directed c-Jun activation/stabilization is independent of JNK.
Article Snippet: .. Transactivating activity of AP-1 was measured at 50–70% confluence by cotransfection of a luciferase expression plasmid containing three repeats of the AP-1 binding site and other expression constructs using cationic liposomes (DAC-30, Eurogentec, Sart Tilman, Belgium). .. Expression constructs Sgn5, Sgn2, and Dc-Jun-(1–226) were cloned into the pcDNA3 vector (Invitrogen) expressing a N-terminal Flag-tagged sequence.

Liposomes:

Article Title: p21-activated Kinase 1 Activates the Nuclear Factor κB (NF-κB)-inducing Kinase-IκB Kinases NF-κB Pathway and Proinflammatory Cytokines in Helicobacter pyloriInfection
Article Snippet: .. Transient Transfections and Reporter Assays—Transactivating activity of NF-kB was analyzed in 293 or HeLa cells by cotransfection of a luciferase expression plasmid (400 ng) containing three repeats of the NF-kB human immunodeficiency virus-binding site and expression constructs using cationic liposomes (DAC-30, Eurogentec). .. 16 h after transfection cells were infected with H. pylori, treated with 10 ng ml21 tumor necrosis factor a (TNFa) (Promega), or left untreated.

Article Title: COP9 signalosome-directed c-Jun activation/stabilization is independent of JNK.
Article Snippet: .. Transactivating activity of AP-1 was measured at 50–70% confluence by cotransfection of a luciferase expression plasmid containing three repeats of the AP-1 binding site and other expression constructs using cationic liposomes (DAC-30, Eurogentec, Sart Tilman, Belgium). .. Expression constructs Sgn5, Sgn2, and Dc-Jun-(1–226) were cloned into the pcDNA3 vector (Invitrogen) expressing a N-terminal Flag-tagged sequence.

Binding Assay:

Article Title: COP9 signalosome-directed c-Jun activation/stabilization is independent of JNK.
Article Snippet: .. Transactivating activity of AP-1 was measured at 50–70% confluence by cotransfection of a luciferase expression plasmid containing three repeats of the AP-1 binding site and other expression constructs using cationic liposomes (DAC-30, Eurogentec, Sart Tilman, Belgium). .. Expression constructs Sgn5, Sgn2, and Dc-Jun-(1–226) were cloned into the pcDNA3 vector (Invitrogen) expressing a N-terminal Flag-tagged sequence.

Recombinant:

Article Title: Identification of the Receptor Subtype Involved in the Analgesic Effect of Neurotensin
Article Snippet: .. Stable expression of the NTR2 into CHO cells was obtained by transfection with 5 mg of recombinant pcDNA3 vector using 25 mg of the cationic liposome DAC-30 (Eurogentec) according to the manufacturer’s recommendations. .. Clonal cell lines were selected by Geneticin (Sigma) (0.5 mg/ml) and isolated before determination of expression level.

other:

Article Title: Antisense oligodeoxynucleotides against thrombomodulin suppress the cell growth of lung adenocarcinoma cell line A549.
Article Snippet: Correspondence: Oichi Kawanami, MD, Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School, 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa 211-8533, Japan.. Email: kawanami@nms.ac.jp.. Received 6 September 2001.

Article Title: Identification of the Receptor Subtype Involved in the Analgesic Effect of Neurotensin
Article Snippet: Stable expression of the NTR2 into CHO cells was obtained by transfection with 5 μg of recombinant pcDNA3 vector using 25 μg of the cationic liposome DAC-30 (Eurogentec) according to the manufacturer’s recommendations.



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